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Figure 4


Fig. 4. Enteric neuron migration under influence of neurochemicals affecting heme oxygenase catalyzed CO production. Immunofluorescence images of enteric neuron migration on individual guts after 24 hours in vivo culture. Dissected guts were stained with cGMP antiserum. Composed images are resulting from microphotographs of several focal planes, that were converted to gray scale, inverted for fluorescence intensity and arranged in Adobe Photoshop. (A) Control conditions. (B) Inhibition of HO enzymes by 5 µM ZnBG. (C) Activation of HO with excess of its substrate analogue hemin (100 µM). (D) Exogenous carbon monoxide (CORM-II, 20 µM). Broken lines indicate foregut-midgut boundary. Arrows indicate furthest migrated enteric neuron. ig, ingluvial ganglion. Scale bar: 500 µm.





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