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Fig. 5. Western blot analysis of HO in the enteric nervous system.
(A) Immunoblots obtained from whole guts at 65% E with enteric nervous
system attached (ENS) and from dissected CNS. HO-2 antibody recognizes
proteins of 
30-35 kDa. In CNS and cytosolic fractions an additional,
slightly heavier protein band is enriched in the membrane fraction of
homogenates. Both membrane (left) and cytosolic (right) fractions derive from
the same preparation. Bottom lanes provide an acetylated 
-tubulin band
as loading control. For quantification, the ratio of the HO-2 signal to the
-tubulin signal was calculated for each lane (n=3, s.e.m. of
the ratios are: ±0.29 membrane ENS, ±0.17 membrane CNS,
±0.08 cytosol ENS, ±0.03 cytosol CNS). (B) Immunoblotting
of recombinant rat HO-2 protein and membrane fraction of ENS at 65% E. The
blots were probed either with the antibody against HO-2 (left, anti-HO-2) or
corresponding antibody solution pre-adsorbed with HO-2 protein (right,
anti-HO-2, pre-adsorbed). Arrowheads in A and B indicate distinct HO
bands.
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