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Fig. 6. Immunocytochemical detection of HO in the enteric nervous system.
(A,B) Immunocytochemical labeling of the enteric neurons
migrating on the midgut at 65% E, using the antiserum in a whole-mount (A) and
tissue blot (B) preparation. 1% BSA was added to the blocking solution in B.
(C) HO-2 immunocytochemistry at 95% E on a whole-mount midgut. Enteric
neurons have left the four migratory pathways and established the midgut
plexus. At this stage, other tissues such as gut musculature show similar
labeling intensity. (D) ENS at the same developmental stage as in C but
stained for acetylated
-tubulin. A, C and D are composed images from
microphotographs of several focal planes. m, gut musculature. Scale bars: 50
µm.