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Figure 6


Fig. 6. Immunocytochemical detection of HO in the enteric nervous system. (A,B) Immunocytochemical labeling of the enteric neurons migrating on the midgut at 65% E, using the antiserum in a whole-mount (A) and tissue blot (B) preparation. 1% BSA was added to the blocking solution in B. (C) HO-2 immunocytochemistry at 95% E on a whole-mount midgut. Enteric neurons have left the four migratory pathways and established the midgut plexus. At this stage, other tissues such as gut musculature show similar labeling intensity. (D) ENS at the same developmental stage as in C but stained for acetylated {alpha}-tubulin. A, C and D are composed images from microphotographs of several focal planes. m, gut musculature. Scale bars: 50 µm.





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