DEV033290 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Distribution of various glycans in wild-type and twd^rw685 embryos. Vagus motor neurons and glycans are detected by anti-GFP antibody, and the biotin-conjugated lectins or zn12 antibody. Lens culinaris agglutinin (LCA; A,B), wheat-germ agglutinin (WGA; C,D), Ricinus communis agglutinin I (RCA-I; E,F), zn12 antibody (G,H), Ulex europaeus agglutinin I (UEA-I; I,J). Wild-type (A,C,E,G,I) and twd^rw685 (B,D,F,H,J) embryos were fixed at 2 dpf and stained.

• Movie 1 -

  Movie 1. Time-lapse movie showing the migration and accumulation of vagus motor neuron progenitors in hindbrain explants. Composite stacks of serial optical images were made every 15 minutes at 24-48 hpf of wild-type embryos. Cross-section views are shown, with dorsal towards the top.

• Movie 2 -

  Movie 2. Time-lapse movie showing the migration and accumulation of vagus motor neuron progenitors in hindbrain explants. Composite stacks of serial optical images were made every 15 minutes at 24-38 hpf of twd^rw685 mutant embryos. Cross-section views are shown, with dorsal towards the top.

• Movie 3 -

  Movie 3. Time-lapse movie showing the migration and accumulation of vagus motor neuron progenitors in hindbrain explants.Composite stacks of serial optical images were made every 15 minutes at 24-38 hpf of twd^rw685 mutant embryos injected with L-GMDS mRNA. Cross-section views are shown, with dorsal towards the top.