DEV028373 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Electroporation of the caudal-most NT of E4/HH24 chick embryos with a control GFP-coding plasmid. (A) Whole-mount GFP expression caudally to somite 47 (s47), 24 hpe. (B,C) Cross-sections double labeled with HNK1 and GFP (B,C) and hybridized for Sox10 expression (B′,C′). Note the presence of GFP-positive cells that are HNK1⁺ and Sox10⁺. Scale bars: 50 µm.

• Supplemental Figure S2 -

  Fig. S2. Msx1, Nog and Wnt1 expression in the caudal-most NT of E5/HH26 chick embryos. (A-C′′) Whole-mount ISH to detect Msx1 (A,A′), Nog (B,B′) and Wnt1 (C,C′) in dorsal (A,B,C) and lateral (A′,B′,C′) views, and cross-sections caudally to the level of somite 47 (s47) (A′′,B′′,C′′). Note that Msx1 and Wnt1 are lacking in the dorsal caudal-most NT, in contrast to Nog, which is still present. Scale bars: 50 µm.

• Supplemental Figure S3 -

  Fig. S3. Electroporation of the trunk NT of E2/HH11-12 chick embryos with a control GFP-coding plasmid. Whole-mount GFP expression at 8 hpe (A) and ISH for Sox10 (B). Cross-sections of the whole mount showing migration of NCCs at dissociating (B′) and epithelial (B′′) somite levels. Scale bars: 50 µm.