DEV034157 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Gli1 expression at E9.0 and E10.0. (A-F) Gli1 expression visualized by in situ hybridization on wild-type E9 (A-C) and E10 (D-F) embryos. Gli1 is expressed in the splanchnic mesoderm but not in the heart. RT-PCR demonstrates Gli1 expression in the splanchnic mesoderm and the neural tube, but not in the heart at E9.0 and E10.0. H, heart; DM, dorsal mesocardium; SM, splanchnic mesoderm; At, atrium; LV, left ventricle; RV, right ventricle; OFT, outflow tract.

• Supplemental Figure S2 -

  Fig. S2. Hh signaling is not required for cell survival or proliferation in the DMP. (A,B) LysoTracker Red analysis shows no difference in the level of cell death in the DMP of control embryos (A) or Smo^Gli1-CreERT2 mutant littermates (B) at E10.5 (sagittal views, 10×). (C-F) Sagittal sections from E10.5 control embryos (C,E) and Shh^-/- mutant littermates (D,F) treated with BrdU 5 and 2.5 hours prior to dissection. There are numerous proliferating cells in the DMP of both control and mutant embryos, with no evident difference in the number of proliferating cells between the two (C,D 6×; E,F 10×). Arrowhead: pulmonary endoderm. At, atrium; V, ventricle; OFT, outflow tract; DMP, dorsal mesenchymal protrusion.