DEV032920 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Neuropilin gene dosage effects during PNS segmentation. (A-F) Longitudinal sections through E10.5 (A,B,E,F) and E11.5 (C,D) trunks stained for neural-specific β-tubulin (TuJ1). (A) Nrp1^Sema−/−;Nrp2^+/− (n=3) and (B) Nrp1^Sema+/−;Nrp2^−/− DRGs are segmental at E10.5 (n=5). (C) DRGs are segmental in Nrp1^Sema−/−;Nrp2^+/− at E11.5 (n=3). (D) Nrp1^Sema+/−;Nrp2^−/− DRGs are segmental but occasionally fused at E11.5 (n=4). (E,F) Longitudinal sections through E10.5 trunks. Top image in each panel shows the initial motor axon projections out of the spinal cord in a dorsal section, whereas the bottom image shows the fasciculation of the same projections in a more ventral section through the same embryo. (E) Nrp1^Sema−/−;Nrp2^+/− motor axons initially project uniformly into the somite and are defasciculated within the anterior sclerotome. (F) Nrp1^Sema+/−;Nrp2^−/− motor axons project along the entire length of the spinal cord, but fasciculate, occasionally in multiple bundles, into ventral roots restricted to the anterior sclerotome. a, anterior; drg, dorsal root ganglia; p, posterior; sc, spinal cord. Scale bar: 50 µm.

• Supplemental Figure S2 -

  Fig. S2. Normal somite polarity in neuropilin double-mutant embryos. (A-D) Longitudinal sections through embryos in situ hybridized for Uncx4.1. Anterior is to the left. Uncx4.1 is expressed by posterior sclerotome cells at E9.5 in both (A) wild-type and (B) Nrp1^Sema−/−;Nrp2^−/− embryos. This restriction to the posterior sclerotome is maintained through E10.5 in both (C) wild-type and (D) neuropilin double-mutant embryos. a, anterior; dm, dermamyotome; nt, neural tube; p, posterior; sc, spinal cord; scl, sclerotome. Scale bars: 100 µm.