DEV031369 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Flow chart of preparation of EpCAM⁺ and EpCAM⁻ cells from livers by two-step selection. For the in vitro colony formation assay, two-step selection was performed. Because the percentage of EpCAM⁺ cells per total NPCs is very low (∼1% or less) in both normal and DDC liver, cell sorting by FACSVantage takes many hours. To save time and reduce the damage to cells, cell sorting was performed following selection by immunomagnetic beads (AutoMACS). The number of EpCAM⁺ cells per liver was estimated based on their percentage in recovered cells after AutoMACS, as the low percentage of EpCAM⁺ cells per total NPCs would otherwise introduce some errors.

• Supplemental Figure S2 -

  Fig. S2. Flow chart of the screening procedure for cell surface marker genes of rat and mouse oval cells.

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF