DEV022426 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1: Comparison of single-sided SPIM and mSPIM. Multidirectional selective plane illumination microscopy (mSPIM) dramatically reduces absorption and scattering artifacts and provides an evenly illuminated focal plane, as demonstrated for a 32 hpf zebrafish embryo. Selected frames are shown in Fig. 5B and 5C.

• Movie 2 -

  Movie 2: Vascular endothelial cell dynamics at long time scales. Dynamics in cell migration are captured with high-speed SPIM time-lapse recordings monitoring the sprouting of a vessel (arrow) in the head of a 24 hpf zebrafish. Tg(flk1:GFP)^s843 expression labels the endothelial cells and Tg(gata1:DsRed)^sd2 expression labels the red blood cells. Shown are maximum intensity projections of a 3D stack. Selected frames are shown in Fig. 6B.

• Movie 3 -

  Movie 3: Vascular endothelial cell dynamics at short time scales. Dynamics in cell migration are captured with high-speed SPIM time-lapse recordings. Highly dynamic protrusions in vascular endothelial cells labeled by Tg(flk1:ras-Cherry)^s896 expression in a 32 hpf zebrafish. A 3D stack was acquired every minute. Selected frames are shown in Fig. 6C.

• Movie 4 -

  Movie 4: High-speed recordings of zebrafish heart beats. High-speed mSPIM video sequence of a transgenic fish expressing fluorescent proteins in the endocardium Tg(flk1:GFP)^s843, myocardium Tg(cmlc2:DsRed)^s879 and blood Tg(gata1:DsRed)^sd2. The movie was recorded at 69 frames per second. Selected frames are shown in Fig. 7.

• Movie 5 -

  Movie 5: Dendrite pruning in class IV da neurons in Drosophila. Drosophila pupae can be imaged in SPIM over many hours. Dendrite pruning of one ddaC neuron (outlined in Fig. 8B) during the course of almost 10 hours. Selected frames are shown in Fig. 8.