DEV033712 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. CA ablation delays larval development and reduces body weight. To understand whether JH biosynthesis within the CA regulates Drosophila larval development, we measured lethality, the period from egg laying to early wandering (W₅₀), and body weight among the four lines: (1) Aug21>; UAS-grim, (2) Aug21>, (3) UAS-grim, and (4) Aug21>; UAS-jhamt at EW. First, all four lines were able to survive to EW. Second, W₅₀ of Aug21>; UAS-grim was delayed by ∼20 hours relative to W₅₀ of the other three lines (A) showing CA ablation resulted in delayed larval development. Third, the body weight of Aug21>; UAS-grim was reduced by ∼25% in females and ∼15% in males (B). Fourth, Aug21>; UAS-grim had a normal body length but a reduced body width in third instar larval (C) and pupal (C′) stages.

• Supplemental Figure S2 -

  Fig. S2. More differentially expressed protein and mRNA profiles in the fat body. (A-O′) The fat body protein profiles of the JH-deficient line Aug21>; UAS-grim and the control line Aug21> were compared at three developmental stages (EW, WPP and 6AP) using the 2D-DIGE/MS analysis. Internal standard (IS) is the mean value of the protein in all the fat body samples and is used for normalization. qPCR was used to assess the fat body mRNA profiles of (1) the JH-deficient line Aug21>; UAS-grim, (2) the control line Aug21>, (3) the control line UAS-grim, and (4) the JH-overexpressing line Aug21>; UAS-jhamt at EW, WPP and 6AP. rp49 was used for normalization. ***, P<0.001; **, P<0.01; *, P<0.05; ANOVA. For convenience, only the differences of Aug21>; UAS-jhamt at EW and Aug21>; UAS-grim at WPP are shown.