DEV030601 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Diagram of the experimental chamber used for live image acquisition.

• Supplemental Figure S2 -

  Fig. S2. Morphology of axial and paraxial structures in PTK7 mutant embryos II. (A) Montage of 20× confocal images of an immunofluorescently stained Ptk7 mutant embryo (ventral view of the posterior side). (B) Confocal image (40×) of the region in the blue box of A. Note that the wide somites form next to the posterior notochord, far anterior to where they normally form in wild-type embryos (see Fig. 1B). Blue, brachyury; green, fibronectin; red, f-actin. PS, primitive streak; PNC, posterior notochord; S, somite; not, notochord.

• Supplemental Figure S3 -

  Fig. S3. F-actin, myosin IIb and dishevelled II immunostaining in the presomitic mesoderm of wild-type compared with Ptk7 mutant embryos. Single z frames taken at 40×, scale bars: 20 µm. NP, neural plate; not, notochordal plate; PNC, posterior notochordal plate; S, somite; PS, primitive streak.

• Movie 1 -

  Movie 1. Wild-type cell tracking in region corresponding to section 1 (primitive streak) of Fig. S3. The movie is projected from two confocal sections taken 9 µm apart, frames taken every 2 minutes. Anterior, up; posterior, down.

• Movie 2 -

  Movie 2. Wild-type cell tracking in region corresponding to section 2 (posterior presomitic) of Fig. S3. The movie (taken at 20×) is projected from three confocal sections taken 4 µm apart, frames taken every 2 minutes. Anterior, up; posterior, down.

• Movie 3 -

  Movie 3. Wild-type cell tracking in region corresponding to section 3 (anterior presomitic) of Fig. S3. The movie is projected from six confocal sections taken 4 µm apart, frames taken every 2 minutes. Anterior, up; posterior, down.

• Movie 4 -

  Movie 4. Ptk7 mutant cell tracking represents behavior of mutant cells in all regions corresponding to wild type (see Fig. S3). The movie is projected from three confocal sections taken 4 µm apart, frames taken every 2 minutes. Anterior, up; posterior, down.