DEV035444 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. BRX gain-of-function effects on root gravitropism. (A-C) Seedling primary root tip reorientation in gravitropism assays, 24 hours after a 90° change of the gravitropic vector in brx single and brx brxl1 double mutants (A), and in transgenic lines expressing BRX under control of the root cap-specific RCP1 promoter (B) or the constitutive 35S promoter (C). brx^C, introgression of the Uk-1 brx loss-of-function allele into the Col-0 background; brx^S, introgression of the Uk-1 brx loss-of-function allele into the Sav-0 background. Asterisks indicate t-test significance as compared with wild-type control (*, P<0.05; **, P <0.01; ***, P <0.001). Bars indicate the percentage of seedlings that have reoriented their root tips for a given window of degrees (15 degree windows). Sample size was ≥35 seedlings.

• Movie 1 -

  Movie 1. BRX-GFP plasma membrane-to-nucleus trafficking in response to BFA treatment. Movement of BRX-GFP from the plasma membrane to the nucleus (note cells on upper right and lower left) in response to BFA treatment (50 µM BFA added at timepoint zero, after 2 hours of MG132 treatment to obtain observable levels of BRX-GFP). The movie runs from t=0 to t=30 minutes. Arrows point to the nuclei of two cells.