DEV034421 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Cellularization in embryos with altered N/C ratio. (A) Cellularization of wild-type and mh haploid embryos marked by myosin. (B) Schematic of crosses for compound stocks C(2)v/C(3)se, C(1)DX × w/Y, and C(2L);F(2R) × C(2)EN. Owing to male non-disjunction in C(2)v and C(3)se, embryos acquiring extra chromosome arms are also produced. (C) Cellularization initiates earlier in patches with a lower nuclear density (arrowhead) than in those with a higher nuclear density (black arrow). Asterisk indicates the patchy region with a higher nuclear density.

• Supplemental Figure S2 -

  Fig. S2. Reproducibility of the microarray data. (A,B) Scatter plots of expression for replicates at one data point (early cycle 14), which show little variability. (C) Scatter plot of expression for early diploid cycle 14 against early haploid cycle 14 shows differential gene expression.

• Supplemental Figure S3 -

  Fig. S3. Confirmation of the microarray data. (A,B) In situ hybridization for (A) N/C ratio- and (B) time-dependent genes on both wild-type and mh haploid embryos. Expression profiles of each gene from the microarray data are shown above.

• Supplemental Figure S4 -

  Fig. S4. Expression of N/C ratio-dependent genes in haploid embryos. (A-D) In situ hybridization of N/C ratio-dependent genes on ssm haploid embryos.

• Supplemental Figure S5 -

  Fig. S5. Expression of N/C ratio-dependent genes in embryos with compound chromosomes. (A-D) In situ hybridization for N/C ratio-dependent genes on embryos from compound stocks.

• Supplemental Figure S6 -

  Fig. S6. Coupling of transcription of N/C ratio-dependent genes to mitotic cycles. In situ hybridization for the N/C ratio-dependent genes odd and frs, and the time-dependent gene ilp-4 on wild-type and patchy (produced from compound stocks) embryos. Arrows indicate the boundary of mitotic patches. Asterisk indicates the patchy region with a higher nuclear density.