DEV032649 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables S1-S2 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Comparison of Dscr5 protein sequences in different species and the expression pattern of zebrafish dscr5 RNA. (A) Alignment of Dscr5 protein sequences from different species. The two putative transmembrane domains are underlined. (B) Analysis by in situ hybridization of zebrafish dscr5 expression at different early stages. A corresponding section is shown on the right side of each whole-mount embryo.

• Supplemental Figure S2 -

  Fig. S2. Overexpression of dscr5 impairs CE movements in Xenopus. (A) A control embryo at stage 30. (B) A dscr5-injected embryo at stage 30 equivalent. (C) Control animal cap explants cultured to stage 20 equivalent. (D) Uninjected explants treated with activin. (E) Explants overexpressing dscr5 and treated with activin. (F) RT-PCR analysis of the expression of Xbra, Chordin and Wnt8 at stage 10.5. ODC is a loading control.

• Supplemental Figure S3 -

  Fig. S3. Inhibition of JNK activity by gly4ΔC. The AP1-luciferase reporter assay was performed in Xenopus early gastrula ectodermal explants. The result was obtained from five independent experiments (P<0.001, Student’s t-test). Error bars indicate standard deviation.

• Supplemental Figure S4 -

  Fig. S4. Knockdown of dscr5 leads to Dsh-GFP degradation. Embryos were injected with the indicated RNA or MO, and green fluorescence was observed at the shield stage. (A) A Dsh-GFP-injected embryo. (B) An embryo injected with dscr5MO and Dsh-GFP. (C) Rescue of Dsh-GFP expression by mut-dscr5 RNA in a dscr5 morphant. (D) An embryo injected with gly4ΔC and Dsh-GFP RNAs.