Supplemental Figure S1 -
Fig. S1. Hh pathway activity is not affected by loss of mam in wing discs. (A-F) Wing disc clones marked by loss of GFP (green) and stained with Engrailed (red in A,C,D) or ptc-lacZ (red in B,E,F). All discs are oriented with anterior (A) to the left and posterior (P) to the right. (A) Engrailed is expressed in the posterior compartment and in a few cells anterior to the AP compartment boundary (arrow) in response to high levels of Hh. (B) ptc-lacZ is induced by lower levels of Hh in an AP border stripe extending further into the anterior. (A,B) Loss of mam does not alter (A) Hh-induced Engrailed (dashed line indicates a clone) or (B) ptc-lacZ levels in the three clones that cross the ptc-lacZ stripe (arrowheads). (C-F) Engrailed and ptc-lacZ expression are induced similarly in anterior ptc (C,E) or ptc mam (D,F) mutant clones.
