DEV032938 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables S1-S2 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Sequence alignment of the stomatal bHLH genes FAMA, MUTE and SPCH from Arabidopsis, rice and maize. Residues identical among stomatal bHLH gene orthologs are shaded. Gaps are filled with dashes. Red line above sequence marks extent of the bHLH domain and green line indicates the C-terminal domain.

• Supplemental Figure S2 -

  Fig. S2. Schematic representations of FAMA, MUTE and SPCH proteins from Arabidopsis, rice and maize. The placement and extent of specific functional domains are indicated by colored boxes; proteins are positioned with their bHLH domains (orange) in register.

• Supplemental Figure S3 -

  Fig. S3. Overexpression phenotype of 35S::OsSPCH2 in Arabidopsis. (A,B) DIC images of 7-dpg adaxial cotyledons: (A) wild-type, (B) 35S::OsSPCH2. Red arrows indicate extra divisions of pavement cells. Images are shown at the same magnification. Scale bar: 50 µm.

• Supplemental Figure S4 -

  Fig. S4. GUS expression driven by rice OsMUTE promoter in Arabidopsis. (A-D) Expression pattern of the GUS reporter driven by the 5′ regulatory region of OsMUTE. Weak expression is observed in the root tip (A), strong expression in young leaves (B), moderate expression in young inflorescence buds (C) and differential expression among stomatal lineage cells (D) of a 7-dpg cotyledon.

• Supplemental Figure S5 -

  Fig. S5. Quantitation of cell proliferation phenotypes induced by MUTE and OsMUTE expression in Arabidopsis. The number of small (stomatal precursor) cells and pavement cells per 12-dpg adaxial cotyledon in wild-type (WT), MUTE (AL1-2) and OsMUTE (OL1-2) overexpressing lines is shown. Ten cotyledons were scored for each genotype and error bars represent ±s.d.