DEV034892 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Convergent extension defects at bud stage and 48 hours. Injected embryos were scored at bud stage and again at 48 hours to determine the extent of the CE defect. At bud stage, the angle between the tailbud and head was measured. An angle greater than 90 degrees was scored as WT and less than 90 degrees as a CE phenotype. At 48 hours, short embryos with misshapen somites were scored as a CE phenotype. The angle between the head and tailbud in (A) a WT and (B) a morphant embryo. (C) Phenotype of WT (112 embryos), morphant (109 embryos), rescued embryos (121 embryos), cpi-17 (95 embryos) and ca-mlck (121 embryos) injected embryos were scored twice, once at bud stage and again at 48 hours.

• Supplemental Figure S2 -

  Fig. S2. A dose-response range of blebbing and dorsal migration defects in mypt1 morphants. Embryos injected with no morpholino, 0.25, 0.5 or 1.0 ng of mypt1 morpholino. Blebs per hour is plotted on the primary y-axis, and µm per hour of dorsal migration is on the secondary y-axis. The dorsal migration curve is in blue and blebbing in red.

• Movie 1 -

  Movie 1. Movement of WT lateral mesodermal cells at shield stage.

• Movie 2 -

  Movie 2. Movement of WT presomitic mesodermal cells at bud stage.

• Movie 3 -

  Movie 3. Movement of mypt1 MO lateral mesodermal cells at shield stage.

• Movie 4 -

  Movie 4. Movement of mypt1 MO presomitic mesodermal cells at bud stage. Note that blebbing is induced in morphant embryos regardless of cell density.

• Movie 5 -

  Movie 5. Movement of mypt1 MO presomitic mesodermal cells at bud stage treated with Rock inhibitor.

• Movie 6 -

  Movie 6. Movement of ca-rhoA lateral mesodermal cells at shield stage.