DEV034827 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Misexpression of SOX7/17 does not trigger ectopic expression of SOX18 in vivo. (A,B) Ectopic expression of MYC-SOX7 (A) or MYC-SOX17 (B) in cells adjacent to the facio-acoustic neural ganglion complex (red, dashed lines) did not upregulate the expression of SOX18 protein. Endothelial cells from the primary head vein are used as an internal positive control to detect SOX18 expression (green, arrows). Scale bar: 25 µm. d, dorsal; FA, facio-acoustic neural ganglion complex; HV, primary head vein; l, lateral.

• Supplemental Figure S2 -

  Fig. S2. Transgenic Prox1-gfp embryos express GFP in lymphatic endothelial progenitors but not in ectopic sites. (A) Whole-mount immunofluorescence using a GFP antibody revealed the presence of lymphatic endothelial cell precursors in the developing trunk of transgenic Prox1-gfp embryos. (B) GFP and MYC proteins could not be detected in ectopic sites by immunofluorescence on sections of transgenic 10.5 dpc embryos (compare with Fig. 3). Autofluorescence of red blood cells was observed in both green and red channels. Scale bars: 500 µm in A; 50 µm in B,C. FA, facio-acoustic neural ganglion complex; H, heart.

• Supplemental Figure S3 -

  Fig. S3. SOX-F antibody detects all three group F Sox proteins. Western-blot analysis of TM3 cells transfected with Sox3, Sox9, Sox7, Sox17 or Sox18 expression constructs. The SOX-F antibody detected SOX7, SOX17 and SOX18, but not the negative controls SOX3 and SOX9 (upper panel). α-Tubulin was used as a loading control (lower panel).