DEV031971 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF

• Supplemental Table S3 - Adobe PDF

• Supplemental Table S4 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Nucleotide and deduced amino acid sequence of LOF1 gene. Coding region is shown in blue.

• Supplemental Figure S2 -

  Fig. S2. Location of T-DNA and Ds insertions in LOF1. Numbers indicate position relative to the LOF1 translation start site. Arrows indicate gene orientation. Red arrows indicate orientation of the GUS gene in the Ds element insertions in ET4016 and GT12154.

• Supplemental Figure S3 -

  Fig. S3. The relationship of LOF1 to related MYB proteins. An unrooted phylogenetic tree of the seven MYB genes in subgroup 21 (Stracke et al., 2001). Full-length amino acid sequences were aligned in MultiAlin (bioinfo.genopole-toulouse.prd.fr/multalin/multalin.html), followed by manual editing. Phylogenetic analysis was performed using default settings of the TreeTop-Phylogenetic Tree Prediction (www.genebee.msu.su/services). Bootstrap values represent percentage of 100 resampled trees at each tree node.

• Supplemental Figure S4 -

  Fig. S4. Location of T-DNA and Ds insertions in LOF2. Numbers indicate position relative to the LOF2 translation start site. Arrows indicate gene orientation. Red arrows indicate orientation of the GUS gene in the Ds element insertions in ET101 and ET5681.

• Supplemental Figure S5 -

  Fig. S5. Expression of LOF1 and LOF2 overlaps in early organ development but is distinct in mature stages. (A,C) Longitudinal section (A) and cross section (C) of ET4016 apex, reporting LOF1 expression. Arrows indicate GUS expression in early organ boundaries. (B,D) Longitudinal section (B) and cross section (D) of pLOF2:GUS apex. (E) Summary of LOF1 and LOF2 expression in inflorescence apex.

• Supplemental Figure S6 -

  Fig. S6. Accessory shoot elongation after removal of primary or axillary apex in Col. (A) Intact paraclade junction with accessory branch that is not elongated. (B) Paraclade junction 2 weeks after cutting the primary apex. Accessory branch has not elongated. (C) Paraclade junction 2 weeks after cutting the axillary apex; note elongated accessory branch. ac, accessory branch; ax, axillary stem; c, cauline leaf; ps, primary inflorescence stem.

• Supplemental Figure S7 -

  Fig. S7. Boundary gene transcript abundance in dissected paraclade junctions of lof1-1 and wild type. (A,B) RT-PCR analyses of LOF1, LAS, RAX1, LOB, BOP1, LOF2, REV, CUC1, CUC2 and CUC3 transcript levels in paraclade junctions of Col wild-type or lof1-1 plants. RT-PCR products were detected by blotting and probing with gene-specific probes after 20 cycles of amplification. ACT2 was used as a control, with 12 cycles of amplification. Two biological replicates are shown in panel A. (C) RT-PCR analyses of LOF1 using different amounts (1 and 3 µl) of cDNA template demonstrates that PCR reactions are quantitative under these conditions.