DEV036830 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Fgf8 expression marks a contiguous AER in medially displaced hindlimb buds of Wnt5a^−/− embryos. (A) Embryo from Fig. 1J showing Fgf8 expression in the AER of hindlimb buds and extending across the ventral midline. Lines indicate lanes of section shown in B and C. (B) Sagittal section through the ventral body wall showing Fgf8 expression in the ectoderm. The ectodermal cells have a stratified appearance in both B and C. (C) Fgf8 expression in a section through the limb bud and AER.

• Supplemental Figure S2 -

  Fig. S2. The ectodermal-endodermal interface at the cloacal membrane is reduced in Wnt5a^−/− embryos. High magnification lateral views of cloaca in specimens from Fig. 3 show that ectoderm and endoderm remain in contact in Wnt5a^−/− embryos that initiate genital tubercle outgrowth. In comparison to heterozygous littermates (left panel), the region of endoderm-ectoderm contact was smaller in some Wnt5a^−/− embryos (right panel; compare distance between black arrowheads which denote the anterior and posterior limits of the endoderm-ectoderm boundary).

• Supplemental Figure S3 -

  Fig. S3. Expression domains of Dusp6, sprouty 4, Etv5 and Etv4 in E11.5 genital tubercles. Expression of Dusp6, sprouty 4, Etv5 and Etv4 at E11.5 in Shh^GFP-cre;Fgf8^+/− embryos in lateral view to show the anteroposterior expression limits with respect to the Fgf8 domain (compare with Fig. 2). The expression of Dusp6 i

• Supplemental Figure S4 -

  Fig. S4. Expression of Etv4 and sprouty 4 in the genital tubercle is unaffected by removal of Fgf8. (A-C) Etv4 and sprouty 4 expression at E11.5 in control (A) and Fgf8 cKO (B,C) embryos. Etv4 and sprouty 4 show similar expression intensities and domains of expression in the genitalia of Fgf8 mutant and control embryos.