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Figure 4


Fig. 4. A BM reconstitution study using PLC{gamma}2-null mice. (A) Appearance of small intestines (a,b,d,e,g,i) and Lyve1+ intestinal lymphatic vessels (brown; c,f,h,j). a-c, PLC{gamma}2-null BM-reconstituted wild-type mice; d-f, wild-type BM-reconstituted PLC{gamma}2-null mice. b is a higher magnification of a. (d,e) Two representative segments from one mouse. Plcg2+/EGFP (g,h) and Plcg2EGFP/EGFP (i,j) mice not subjected to BM transplantation are shown for comparison. Scale bars: 1 mm. (B) Sections of small intestines of BM-reconstituted mice immunostained for Lyve1 (green) and CD31 (red). The type of transplantation is indicated at the top of each panel. Merged images are shown. Arrows indicate connections between CD31+, Lyve1- blood vessels (red) and CD31- or low, Lyve1+ lymphatic vessels (green/yellow). Scale bar: 100 µm.





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