spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Notch-mediated DeltaD endocytosis results in DeltaD degradation. (A-B') Distribution of DeltaD-HA in WT and Notch MO zebrafish embryos. In vitro synthesized mRNA encoding DeltaD-HA and MycPM was co-injected with control (WT in A,A') or notch1a and notch3 MOs (Notch MO in B,B'). Embryos are labeled with anti-HA mAb (green) and anti-myc pAb (red) at 80% epiboly stage. More DeltaD-HA is localized in cytoplasmic puncta in WT than in Notch MO cells. (C-H') Comparison of DeltaD-HA (green) and MycPM stability with (F-H) and without Notch morpholinos (C-E). DeltaD-HA expression reduces from 80% epiboly (C) to the two-somite stage (2 ss) (D) and is barely detectable at the five-somite stage (5 ss) (E) in WT. By comparison, MycPM expression levels are progressively increased from 80% epiboly to 5 ss (C'-E'). In Notch MO embryos, Delta-HA (F-H) behaves like MycPM (F'-H') and its expression is not decreased.





Right arrow Return to article