DEV029561 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Wnt3 and Wnt8 are expressed in the vicinity of KV. (A-D) wnt3 and wnt8 expression was detected in the bud region of embryos at the bud stage (A,B) and the 12-somite stage (C,D). (E,F) The expression of wnt3 and wnt8 was in the vicinity of KV, as indicated by co-staining with charon. (G) Staining of charon alone at 12-somite staged embryos. Shown are lateral views with anterior to the left.

• Supplemental Figure S2 -

  Fig. S2. The effect of Wnt/β-catenin signaling on cardiac jogging. (A-C) Representative images of cardiac left-jog (L-jog), No-jog and right-jog (R-jog). Shown are dorsal views of 28 hpf embryos after in situ hybridization using cmlc2 as a riboprobe. Anterior is to the top. (D) Quantification of embryos in each category of cardiac jogging. n, total numbers of embryos scored.

• Supplemental Figure S3 -

  Fig. S3. The effect of Wnt/β-catenin signaling on midline development. (A-P) Midline structures were not affected by alterations of Wnt signaling, as revealed by staining for the midline mesendodermal markers axial (A-H) or gsc and ntl (I-P). Embryos were co-stained with pax2 as an anteroposterior reference. Shown are dorsal views with anterior to the top of tailbud-staged embryos. (Q-T) lefty1 expression in the notochord was abolished in wnt3 morphants (T), but not in apc morphants (R) or in embryos injected with wnt3 RNA (S). Shown are dorsal views of 21-somite staged embryos with anterior to the top.

• Supplemental Figure S4 -

  Fig. S4. Gata4 is not required for KV development or midline development. (A-C) The expression of charon was not changed in gata4 morphants (B) or in embryos injected with gata4 RNA (C), when compared with controls (A) at the 12-somite stage. Shown are dorsal views of the tail bud region. (D-F) KV cilia development was not affected in gata4 morphants (E) or in embryos injected with gata4 RNA (F), when compared with controls (D) at the 12-somite stage, as visualized by anti-acetylated tubulin antibody staining; this is also summarized by measurement of cilia length (J) and cilia numbers (K). (G-I) Midline structure was not affected in gata4 morphants (H) or in embryos injected with gata4 RNA (I), as revealed by staining for the midline mesendodermal markers gsc and ntl. Shown are dorsal views of tailbud-staged embryos with anterior to the top. Data shown in J and K are means±s.d. Ten to 22 embryos were scored for each experimental group.

• Supplemental Table S1-S2 - Adobe PDF