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Fig. 3. debcl and genotoxic stress. (A-N) After irradiation, debcl mutants show an elevated incidence of visible defects, but are unaffected for stress-induced apoptosis and cell-cycle arrest. (A,B) Basal levels of cell death are observed in the larval wing imaginal disc. (C,D) Four hours after exposure to 4000 Rads of ionizing radiation (IR), robust cell death is detected by Acridine Orange staining (AO). (M) Adult survival curve after treatment with IR. The corresponding LD₅₀ is shown for each sample (dashed line). Effector caspase levels labeled by -proCaspase3 Ab staining (G) drastically increased after IR challenge in WT and debcl^22/27 tissue (J, WT not shown). Cell proliferation in the larval L3 wing imaginal disc was examined before (H,I) and after (K,L) treatment with 4000 Rads of IR, by -phosphohistone H3 Ab staining. Wing from a WT (yw, E) and debcl^22/27 (F) adult after IR challenge, showing a notched wing phenotype in the debcl mutant. (N) Histogram quantifying the percentage of notched wings in WT (yw) and transheterozygous debcl mutant alleles at 0, 1500 and 2500 Rads of IR. Dashed line represents WT percentage of notched wings.

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