DEV026419 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Effects of gene knockdown. Knocked-down genes are indicated at the lower right of each photograph. The genes examined by whole-mount in situ hybridization are indicated at the left side of photographs. Expression lost in each experimental embryo is shown by white arrows, whereas ectopic expression is indicated by black arrows. Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively. Ciona savignyi embryos, which were used for testing specificities of MOs, are labelled ‘Cs’.

• Supplemental Figure S2 -

  Fig. S2. Gene regulatory network associated with single cells of the posterior neural plate of the late gastrula embryo. Cell names are shown on the top of each panel. Transcription factor genes are indicated by rectangles. Signaling ligand genes are shown by ovals. Arrows indicate transcriptionally regulatory interactions. The flat-headed arrows indicate repression. Genes expressed in the ancestors of the cell, but not expressed at the indicated stage, are enclosed by black rectangles or ovals. Genes that are not expressed in either the specified cell or its ancestors are shown in light gray. Color codes for cells are the same as in Fig. 1.

• Supplemental Figure S3 -

  Fig. S3. Gene regulatory network associated with single cells from the posterior part of the brain to the caudal nerve cord of the early tailbud embryo. Cell names are shown on the top of each panel. Transcription factor genes are indicated by rectangles. Signaling ligand genes are shown by ovals. Arrows indicate transcriptionally regulatory interactions. The flat-headed arrows indicate repression. Genes expressed in the ancestors of the cell, but nor expressed at the indicated stage are enclosed by black rectangles or ovals. Genes that are not expressed in either the specified cell or its ancestors are shown in light gray. Color codes for cells are the same as in Fig. 1.

• Supplemental Figure S4 -

  Fig. S4. Gene regulatory network associated with single cells in the ganglion of the middle tailbud embryo. Cell names are shown on the top of each panel. Transcription factor genes are indicated by rectangles. Signaling ligand genes are shown by ovals. Arrows indicate transcriptionally regulatory interactions. The flat-headed arrows indicate repression. Genes expressed in the ancestors of the cell, but not expressed at the indicated stage, are enclosed by black rectangles or ovals. Genes that are not expressed in either the specified cell or its ancestors are shown in light gray. Color codes for cells are the same as in Fig. 1.