DEV027193 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S2 -

  Fig. S1. Characterization of Olig3 expression in the dorsal alar plate of rhombomere 7. (A) Schematic section through the mouse hindbrain. Alar and basal plate, as well as the ventricular and mantle zones, are indicated. The details displayed in B-G are indicated. Note that similar sectors are shown in B-D as in Fig. 1 and in E-G as in Figs 2 and 3. (B-G) Immunohistological analysis of the dorsal alar plate of E11.5 embryos using antibodies directed against (B) Olig3 and BrdU, (C) Olig3 and Ki67, (D) Olig3 and βIII-tubulin, (E) Olig3 and Foxd3, (F) Foxd3 and Mash1, and (G) Foxd3 and Ptf1a. Note that Olig3 is present in BrdU⁺, Ki67⁺ and βIII-tubulin⁺ cells, indicating that Olig3 is expressed in proliferating progenitors as well as in postmitotic neurons (B-D). dA4 neurons (Foxd3⁺) arise lateral to cells that express Olig3, Mash1 and Ptf1a. Note that F and G show the same section that was stained for Foxd3, Mash1 and Ptf1a; F and G display Foxd3/Mash1 and Foxd3/Ptf1a signals, respectively. Scale bars: 100 mm.

• Supplemental Figure S2 -

  Fig. S2. Gad1 expression in the anterior medulla oblongata of control and Olig3 mutant mice. In situ hybridization analyses using a Gad1-specific probe on sections of the anterior medulla oblongata of control (A) and Olig3 (B) mutant mice. Scale bar: 100 μm.