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Fig. 6. Cooperation of Olig3 and Ptf1a and permissive functions of Olig3 in the
determination of the Foxd3+ dA4 fate. (A-C,E-G)
Chick hindbrains electroporated with mouse Olig3 (A-C,F), Ptf1a (E), Olig3 and
Ptf1a (G). Effects were assessed by immunohistochemistry using antibodies
against Lbx1 (A) or Pax2 (B), or by in situ hybridization using probes
specific for Ptf1a (C) or Foxd3 (E-G). In addition, a GFP
vector was co-electroporated to identify electroporated cells. Note that A and
B show the same section stained with antibodies against Lbx1, Pax2 and GFP; A
and B display the Lbx1/GFP and Pax2/GFP signals, respectively.
(D,H) Quantification of cells that expressed particular
transcription factors in the dorsal part of the chick caudal medulla
oblongata. (I-O) Comparison of the dorsal alar plate in control (I,M),
Olig3 mutant (J,N), Olig3; Lbx1 double-mutant (K,O)
and Lbx1 mutant (L) mice, using antibodies against Foxd3 and Pax2
(I-L) and Foxd3 and Phox2b (M-O). The dashed line indicates the ventral limit
of Oligo3 expression. (P) Summary of neuronal types observed
in control, Olig3 and Olig3; Lbx1 mutant mice. The
colors indicate the neural subtypes defined in
Fig. 1I by their transcription
factor code. Scale bars: 50 µm.
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