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Figure 5


Fig. 5. PP2A counteracts kinases to regulate Ci and Hh target gene expression. (A,A') A wild-type wing disc was immunostained to show CiFL and ptc-lacZ expression. (B,B') A wing disc overexpressing UAS-mC* by MS1096 Gal4. Arrowhead in B shows the downregulated CiFL level and arrowhead in B' shows the attenuated ptc-lacZ expression. (C,C') A wing disc overexpressing UAS-Wdb by MS1096 Gal4. Arrowhead in C shows the upregulated CiFL. (D,D') A wing disc co-expressing UAS-mC* with UAS-Wdb by MS1096 Gal4. Arrowhead in D shows reinstated CiFL and arrowhead in D' shows the partially restored ptc-lacZ expression. (E,F) Wing discs expressing UAS-CRL or co-expressing UAS-CRL with UAS-MtsRNAi by MS1096 Gal4 and stained to show the levels of CiFL.





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