DEV039693 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Formation of complete secondary embryonic axes. (A-D) Formation of a complete secondary axis, extending anteriorly up to the anterior head and containing axial tissue, after injection of noggin1 and frzb RNAs into a ventral blastomere at the 16-cell stage in lateral (A) and frontal (B) views. (C) Detail of the trunk region in lateral view. An identical secondary axis can be obtained upon overexpression of nodal by injection of ndr2 RNA into a ventral blastomere at the 16-cell stage, as shown in lateral view (D).

• Supplemental Figure S2 -

  Fig. S2. Organizer activity of the lateral blastula-early gastrula margin. (A-E) Grafts of various parts of the early gastrula margin from a donor labeled with GFP to the animal pole of a host blastula (unlabeled) result in the organization of a partial ectopic secondary axis that contains labeled (fluorescent, green) cells from the donor as well as unlabeled cells that have been recruited from the animal pole of the host. (A) Graft of the ventral margin. (B) Graft of the lateral margin. (C,D) Grafts of the dorso-lateral margin resulting in the formation of posterior head/anterior trunk structures (C) or posterior head (D). (E) Graft of the dorsal margin resulting in the formation of a secondary axial structure (arrowhead, unlabeled notochord cell). Lateral view, anterior to the left, dorsal to the top. Left panels, live embryo; middle panels, fluorescently labeled cells; right panels, merge of live and florescent images.