DEV042374 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

• Supplemental Figure S1 -

  Fig. S1. Sexual identity is established by stage 15 of development. Percentage of XX or XY Drosophila embryos with gonad in situ hybridization signal for the indicated probes at (A) stage 13, (B) stage 14, (C) stage 15 and (D) stage 17. Solid bars indicate strong staining, shaded bars, medium staining and hatched bars, weak staining. Robust, male-specific germline gene expression is apparent by stage 15. CG9925 is a non-sex-specific germ cell gene used as a control.

• Supplemental Figure S2 -

  Fig. S2. Sexual identity in germ cells outside of the somatic gonad in tre mutants. (A) Graph shows the percentage of XY and XX germ cells exhibiting in situ hybridization staining either inside the somatic gonad (wild type, WT) or outside the somatic gonad (tre^−/−, OG). (B) Graph of normalized germ cell gene expression (see Materials and methods).

• Supplemental Figure S3 -

  Fig. S3. Non-autonomous regulation of germ cell sexual identity. Graph of the percentage of embryos exhibiting in situ hybridization signal in the gonads for the indicated genes. Solid bars indicate strong staining, shaded bars, medium staining and hatched bars, weak staining. Data from CG9253, CG6751, Rs1, RanGap, CG6693 and Klp61F was used to create the normalized average shown in Fig. 3. (A) tra mutants (tra¹/Δtra), (B) UAS-tra^F; tubulin-GAL4, (C) Stat92E mutants, (D) UAS-upd; nanos-Gal4.

• Supplemental Figure S4 -

  Fig. S4. Analysis of sexual identity in ovo, otu, Sxl and stil mutant germ cells. Graph of the percentage of embryos exhibiting in situ hybridization signal in the gonads for the indicated genes. Solid bars indicate strong staining, shaded bars, medium staining and hatched bars, weak staining. (A) ovo^{D1rv23/D1rv23}, (B) otu^17/17, (C) Sxl^fl/Sxl^f4 (germline-specific allele combination), (D) Sxl^fl/Sxl^f1 (affects germline and soma), (E) stil⁴/Df(2R)stil-B.