DEV036624 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

• Supplemental Figure S1 -

  Fig. S1. Schematic map of the pLVTHM vector containing shRNAs that target the luciferase (control) or GLI3 gene.

• Supplemental Figure S2 -

  Fig. S2. Primitive neuroepithelial (NE) cells at day 10 were negative for FOXG1 (A), as examined by immunostaining. After another week, NE cells were positive for FOXG1 (see Fig. 1). They were also positive for OTX2 (B) and LHX2 (C). Blue, Hoechst-stained nuclei. Scale bars: 50 µm.

• Supplemental Figure S3 -

  Fig. S3. Quantitative PCR showed the expression of dorsal-ventral patterning genes during neural differentiation from mouse ESCs. The differentiation medium and conditions are the same as those for human ESCs. Data are expressed as mean6s.d., n=3.

• Supplemental Figure S4 -

  Fig. S4. Quantitative PCR showed the change of WNT3A mRNA expression during neural differentiation from human ESCs. Mean6s.d., n=4.

• Supplemental Figure S5 -

  Fig. S5. Immunohistochemistry showed the expression of NKX2-1 and FOXG1 proteins by neural progenitors after DKK1 and SHH treatment at day 28. All of the NKX2-1⁺ cells were also positive for FOXG1, suggesting their telencephalic ventral identities. Scale bar: 50 µm.