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Fig. 1. Specific expression of RIM-BP3 in the mouse testis. (A) The
RIM-BP3 protein showing the conserved domains and three antigen regions for
raising antibodies. (B) Northern hybridization analysis of the
RIM-BP3 transcript in different tissues as indicated above. The mRNA
blot (Clontech) contains 2 µg mRNA on each lane. The hybridization probe
corresponds to the C-terminal coding sequence (nucleotides 3547-4260).
(C) Western analysis of the RIM-BP3 protein in different tissues as
indicated above. The detection of 
-tubulin serves as a loading control
(lower panel). (D) Western detection of the RIM-BP3 protein in
developing postnatal testis. Upper panel, diagram of stages of mouse
spermatogenesis; PL, preleptotene spermatocytes; L, leptotene spermatocytes;
Z, zygotene spermatocytes; P, pachytene spermatocytes; D, diplotene
spermatocytes; MI, meiosis I; MII, meiosis II; middle panel, western analysis
of RIM-BP3 (0-60 days post partum); bottom panel, Coomassie blue staining of
the protein samples. (E) Western detection of RIM-BP3 in purified male
germ cells. A, type A spermatogonia; B, type B spermatogonia; RS, round
spermatids; E1 and E2, two pools of elongate spermatids; SP, mature sperm; RB,
residual bodies; ST, Sertoli cells. Other abbreviations used are as in D. The
lower panel shows Coomassie blue staining of samples used for the western
analysis.
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