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Fig. 2. Distribution of the RIM-BP3 protein in elongating spermatids.
Testicular germ cells were prepared from adult wild-type mice, and indirect
immunofluorescence staining was performed using polyclonal anti-RIM-BP3 SbcC
(green) and monoclonal anti-
-tubulin (red) antibodies. The slides were
counterstained with DAPI to visualize the nucleus. Testicular germ cells from
RIM-BP3 knockout mice were used as negative controls (see Fig. S2 in
the supplementary material). Scale bar: 10 µm.