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Figure 7


Fig. 7. The Hook1 azh mutant is unable to interact with RIM-BP3. (A) Schematic representation of the wild-type Hook1 and the azh mutant Hook1 protein (Hook1azh) with their polypeptide length indicated. (B) The expression constructs of Flag-RIM-BP3, HA-Hook1 and HA-Hook1azh were co-transfected into HEK-293T cells with various combinations indicated at the top. Whole-cell extracts were immunoprecipitated with the anti-RIM-BP3 (C) or anti-HA antibody, and the precipitated proteins were analyzed by western blotting using anti-HA or anti-RIM-BP3 (C) antibody as indicated.





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