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Fig. 1. Injection of celsr1a and celsr1b morpholinos in
MZord embryos leads to a defective epiboly phenotype during zebrafish
gastrulation. MZord embryos (A,B,C,G,H) or MZord embryos
injected with 0.4 pmoles each of celsr1a and celsr1b
morpholinos (D,E,F,I,J) were visualised at tail-bud stage with markers, as
indicated in the bottom right-hand corner, by in situ hybridisation.
hgg1 was used to indicate the prechordal plate (pcp), ntl
for the prospective notochord (n) and germ ring blastopore margin, and
dlx3 for the anterior edge of the neural plate (A-F). Embryos
were also visualised at 90% epiboly with phalloidin for actin
(G,I) or anti-
-catenin antibody (H,J). An
arrowhead represents the leading edge of the EVL with the actin cable being
formed (I). An arrow indicates the leading edge of deep cells (J). Note that
the deep cells are delayed from the leading edge of the enveloping layer in
the mutant/morphant embryos. y, yolk. (K) Cell elongation of the EVL
along the animal-vegetal (AV) axis and the mediolateral (ML) axis at 90%
epiboly is quantified as AV/ML ratio (see the inset of G), and the measurement
is made using ImageJ from 80 cells (four embryos of each group). Cells that
are attached with the actin cable are expressed as `Row1', whereas cells that
are not attached to that as `Row2/3'. Means and standard deviations (s.d.) are
shown, and an asterisk indicates a statistically significant difference
(P<0.05; Student's t-test).
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