spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Differential cell cohesive properties of wild-type cells compared with cells from celsr mutant/morphants and cells from embryos expressing Celsr-{Delta}C. Wild-type and MZord embryos were injected with RNA/morpholinos together with fluorescein-dextran (FDX, green) or rhodamine-dextran (RDX, red), as indicated. (A) 30-40% epiboly embryos were dissociated and dissociated cells from different populations were mixed in a minimal volume of a hanging drop and kept overnight to analyse aggregates by confocal microscopy. (B) Cells from wild-type embryos (green) and wild-type embryos (red). (C) Cells from MZord embryos injected with 0.4 pmoles each of celsr1a and celsr1b morpholinos (green) and wild-type embryos (red). (D) Cells from wild-type embryos injected with 300 pg Celsr-{Delta}C-HA RNA (green) and wild-type embryos (red). (E) Cells from wild-type embryos injected with 100 pg Lyn-Celsr RNA (green) and wild-type embryos (red). Celsr mutant/morphant cells (C) or Celsr-{Delta}C-expressing cells (D) are strongly segregated from wild-type cells, whereas Lyn-Celsr-expressing cells are only weakly segregated from wild-type cells (E).





Right arrow Return to article