|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Netrin 1 binding to Dcc recruits Fyn. (A) Increased
interaction between Dcc and Fyn is detected upon addition of exogenous netrin
1 (200 ng/ml, 5 minutes) to cultures of rat oligodendrocytes. This interaction
was detected in both immature (4 DIV) and mature (6 DIV) oligodendrocytes and
was observed when co-immunoprecipitations (IP) were performed with antibodies
against Dcc or Fyn. A constitutive interaction between FAK and Dcc was also
detected. IgG indicates immunoprecipitation with species-matched non-immune
control IgG. (B) Increased active Src family kinase [phospho (p) SFK
(pY416)] associated with Dcc in oligodendrocytes following application of
netrin 1 (200 ng/ml, 5 minutes). (C) No detectable change in SFK
activity in whole-cell lysates of cultured oligodendrocytes treated with
netrin 1 (200 ng/ml) for either 5 minutes or 24 hours. (D)
Quantification of the interaction between Fyn and Dcc, and between active SFK
and Dcc. *P<0.05 versus control. Error bars indicate
s.e.m.
|
