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Fig. 6. Wnt3a treatment rescues the phenotype of Col2.3-11βHSD2
transgenic mice. (A-C) Representative Micro-CT images of the
calvaria of 3-day-old (p3) wild-type and Col2.3-11βHSD2 transgenic mice.
(A) Wild-type vehicle control; (B) Col2.3-11βHSD2 transgenic littermate,
vehicle control; (C): Wnt3a-treated Col2.3-11βHSD2 transgenic mouse
(littermate of A,B). (D) Quantitation of unmineralized suture areas.
Results are expressed as percent of total skull area. Micro-CT images were
analysed using Image J image analysis software. Data are represented as
mean±s.e.m. (*P<0.05;
**P<0.01 versus transgenic, n=8). tg,
transgenic mice; tg Wnt3a, transgenic mice receiving recombinant Wnt3a
treatment. (E-M) Representative coronal sections of parietal bones.
(E,H,K) Wild-type vehicle control; (F,I,L) Col2.3-11βHSD2 transgenic,
vehicle control (CTR) littermate; (G,J,M) Col2.3-11βHSD2 transgenic,
Wnt3a-treated littermate. Toluidine Blue stain. (E-G) Parietal bones, base
segment. Arrows in F indicate areas of remnant cartilage, whereas arrows in E
and G indicate the corresponding positions in wild-type vehicle control and
Wnt3a-treated Col2.3-11βHSD2 transgenic littermates. Scale bars: 400
µm. (H-J) Parietal bone, sagittal suture area. Red arrowheads indicate the
position of active bone expansion (`growing bone fronts'). b, bone. Ectopic
cartilage is present in the sagittal sutures with greater separation of
parietal bones in Col2.3-11βHSD2 transgenic vehicle controls (I) compared
with the wild-type vehicle control mice (H). In Wnt3a-treated transgenic mice,
this phenotype is rescued with only a small cartilage remnant remaining (J).
Scale bars: 100 µm. (K-M) Enlarged suture area of H-J. Red arrowheads
indicate the position of growing bone fronts. b, bone. Parietal bones are well
formed in Wnt3a-treated transgenic mice (M), comparable with what is seen in
wild-type animals (K). By contrast, bone is thin and osteoblasts are
disorganized in transgenic vehicle control mice (L). Scale bars: 100
µm.
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