DEV027599 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Cell-cycle timing of syncytial blastoderm divisions. Representative still DIC micrographs from an imaging sequence of a wild-type embryo illustrate the criteria used to score the onset of interphase and mitosis. Nuclear envelope ‘rings’ (arrowheads) are readily visible at the cortex of the embryo during interphase but not mitosis. (A) Nuclear envelope formation (NEF) marks the onset of interphase 11. (B) Nuclear envelope breakdown (NEB) marks the onset of mitosis 11. (C) NEF marks the onset of the following interphase 12. Images were captured at 20-second intervals and cell-cycle timing was determined by counting frame numbers between NEF and NEB. Timing data were independently validated by a second observer. Strict temperature control (22.0±0.5°C) was maintained throughout the experiment as monitored by a sensor on the microscope stage; wild-type and mutant embryos were alternately analyzed in every recording session as a further control. Time (s, seconds) elapsed from the onset of cycle 11 is indicated for each micrograph. Scale bars: 10 µm.

• Supplemental Figure S2 -

  Fig. S2. NOPO mediates a subset of the functions of BEN. Wild-type, ben and nopo males were compared using several assays. (A) Visually-mediated jump response assay. (B) Climbing assay. (C) Assessment of TDT muscle attachment sites. (D) Innate immunity assay. diptericin induction was measured after injection of buffer (white bars) or E. coli (black bars). Relish (Rel) flies with defective immune response were used as a control. Df is Df(2R)Exel7153. Error bars represent s.e.m. Single, double and triple asterisks represent P-values of <0.05, <0.01 and <0.001, respectively.