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Figure 2


Fig. 2. CG5140 is the nopo gene. (A) nopo structure. Coding regions are represented by black boxes, 5'- and 3'-UTRs by white boxes, splicing events by lines. Arrows indicate transcription direction. Asterisk marks position of E11K mutation in nopoZ1447. Triangles represent P-elements. EYG5845 imprecise excision generated nopoExc142 (gap represents deleted region). Dashed line represents genomic region used to create rescue construct. (B) Comparison of Drosophila NOPO and human TRIP. Gray boxes represent RING domains. Asterisk marks mutation in nopoZ1447. Line indicates NOPO region used for antibody production. (C) Alignment of RING domains of putative NOPO/TRIP homologs in Drosophila melanogaster, Homo sapiens, Mus musculus, Danio rerio, Gallus gallus and Anopheles gambiae. Residues 6-46 of Drosophila NOPO are shown. Crucial RING-domain cysteines and histidines are highlighted. Asterisk marks residue mutated in nopoZ1447. (D,E) NOPO immunoblots. (D) NOPO levels in embryos (1-2 hours) of wild-type or nopo females. Anti-NOPO antibodies recognize a specific band the predicted size of NOPO (48 kDa) and a non-specific band (bg). (E) NOPO developmental western. Anti-GAPDH or anti-{alpha}-tubulin was used as a loading control.





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