spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. NOPO/TRIP, BEN and UEV1A interactions and co-localization. (A) Yeast two-hybrid assay. Yeast cells expressing combinations of NOPO, BEN and UEV1A fused to the Gal4 DNA-binding domain (BD, `bait') or activation domain (AD, `prey') were spotted onto selective media. Growth on SC-Trp-Leu-His media (shown) indicates physical interaction between the fusion proteins. Wild-type and mutant versions of NOPO and BEN (E11K and P97S, respectively) were tested. A representative plate spotted in duplicate is shown; identical results were obtained for three independent Trp+Leu+ colonies per plasmid combination tested. (B-F) Immunofluorescence microscopy of transfected HeLa cells. eGFP-BEN is in green, mCherry-TRIP in red, and DNA in blue. eGFP-BEN (B) and mCherry-TRIP (C) localize distinctly when transfected alone. (D-F) Co-transfection of eGFP-BEN (D) with mCherry-TRIP (E) promotes its localization into nuclear puncta (F, merge). Scale bars: 20 µm.





Right arrow Return to article