DEV027748 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Normal expression of Fgf8 and Shh in the Sall mutant limb. Distal (A,C) and dorsal (B-F) views of E10.5 forelimb bud stained with Fgf8 (A-D) and Shh (E,F), with the anterior towards the top. Wild-type (A,B,E) and Sall1^-/-;Sall3^-/- (C,D,F) limbs are shown. Fgf8 expression and Shh expression appear normal in the Sall1^-/-;Sall3^-/- limb.

• Supplemental Figure S2 -

  Fig. S2. Normal expression of Sall4, Tbx5 and Tbx4 in the Sall mutant limb. Dorsal views of an E10.5 forelimb bud (A-D) and hindlimb bud (E,F) stained with Sall4 (A,B), Tbx5 (C,D) and Tbx4 (E,F), with the anterior towards the top. Wild-type (A,C,E) and Sall1^-/-;Sall3^-/- (B,D,F) limbs are shown.

• Supplemental Figure S3 -

  Fig. S3. Normal expression of Sall1 and Sall3 in the Tbx5 mutant limb. Dorsal views of an E11.0 forelimb bud stained with Sall1 (A,B) and Sall1 (C,D), with the anterior towards the top. Wild-type (Tbx5^+/+; A,C) and Tbx5^+/- (B,D) limbs are shown.

• Supplemental Figure S4 -

  Fig. S4. Normal expression of Hox genes in the absence of Sall1 and Sall3. Dorsal views of E11.5 forelimb buds (A-H) and hindlimb buds (I,J), stained with Hoxa11 (A,B), Hoxa13 (C,D), Hoxd11 (E,F), Hoxd12 (G,) and Hoxd13 (I,J) with the anterior towards the top. Wild-type (A,C,E,G,I) and Sall1^-/-;Sall3^-/- (B,D,F,H,J) limbs are shown. No apparent changes in the expression of these Hox genes are detected. Slightly smaller expression domain of Hoxa13 (D, compare with C), Hoxd12 (H, compare with G) and Hoxd13 (J, compare with I) was detectable.