DEV028365 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Sequence alignment of the four D6PKs. The characteristic DFG to DFD substitution in the conserved catalytic subdomain VII and the specific insertion between subdomains VII and VIII are underlined. The alignment was obtained with the MegAlign Software of the DNAStar Lasergene package using default settings.

• Supplemental Figure S2 -

  Fig. S2. Promoter-GUS expression analyses of the four D6PKs with a special emphasis on expression in the seedling and the seedling root. (A-L) GUS staining of 7-day-old seedlings (A,D,G,J), root tips (B,E,H,K) and lateral root initiation sites (C,F,I,L). Scale bars: 3 mm for A,D,G,J; 1 mm for B,C,E,F,H,I,K,L.

• Supplemental Figure S3 -

  Fig. S3. d6pk mutants and YFP:D6PK transgenic lines have efficient auxin responses. (A) RT-PCR gene expression analysis of auxin-induced gene expression of several AUXIN (INDOLE ACETIC ACID; IAA) genes in 7-day-old wild type, d6pk d6pkl1 d6pkl2 (d6pk012), and YFP:D6PK seedlings treated for 1 hour with the synthetic auxin 2,4D reveals efficient auxin-induced gene expression in the three genotypes. (B) Auxin-induced morphological changes, such as arrest of primary root growth and induction of root hair formation, occur in all three genotypes. (C) Induction of lateral root formation in wild type, d6pk d6pkl1 d6pkl2, and YFP:D6PK after treatment with the auxins NAA and 2,4D. Arrowheads point at lateral roots and lateral root primordia; note the irregular lateral root formation in the d6pk d6pkl1 d6pkl2 mutant after NAA treatment (central panel).

• Supplemental Figure S4 -

  Fig. S4. Differential BFA sensitivity in d6pk mutants and YFP:D6PK seedlings. Seedlings were germinated and grown on the respective BFA concentrations. Photographs were taken of 7-day-old seedlings, when gravitropism defects are not yet pronounced in the d6pk d6pkl1 d6pkl2 mutants.

• Supplemental Table S1 - Microsoft Excel