DEV029868 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1-S2 -

• Supplemental Figure S1 -

  Fig. S1. Sun1 is required for the development of reproductive organs in mice. (A) Analysis of the expression of Sun1 in somatic tissues and reproductive organs in adult mice by semi-quantitative RT-PCR. The expression of Sun1 was detectably higher in mouse testis than in somatic organs. Gapdh expression was used for normalization. (B) The gross appearance and body weight of Sun1^+/+ and Sun1^−/− mice. No apparent differences were observed. (C) The gross appearance of 4-month-old Sun1^+/+ and Sun1^−/− female reproductive organs. (D) TUNEL assay for 4-week-old Sun1^+/+ and Sun1^−/− mouse testis. Sun1^−/− germ cells frequently showed TUNEL-positive staining. (E) H&E staining of ovaries from 4-week-old Sun1^+/+ and Sun1^−/− mice. Growing oocytes were abundantly seen in Sun1^+/+ female mice, but oogenesis in Sun1^−/− animals was not observed.