DEV034017 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables S1-S3 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Wing disc in which the posterior compartment is mutant for dpp, as indicated by the loss of GFP staining. The double label with Engrailed (red), which marks the entire posterior compartment, shows that virtually all the posterior cells are mutant for dpp, except the small remaining patches (arrows).

• Supplemental Figure S2 -

  Fig. S2. Ability of hep^act to induce apoptosis and wingless expression. (A) Wing disc of genotype spalt-Gal4>UAS-GFP showing the wing pouch domain (green) defined by the Gal4 line in relation to the wg domain (blue). (B,C) Disc of genotype spalt>hep^act stained for Caspase activity (red) and Wingless (blue). This disc contains a normal dose of dronc so that cells can enter apoptosis. Because the Gal4 line is acting late in development, it is possible to detect both Caspase and Wg activity in apoptotic cells before they disappear. Note that the Wg protein is clearly visible in the cells with lower levels of caspase on the right of the spalt domain, whereas Wg protein has almost disappeared on the left side, where the cells have higher caspase levels. (D) spalt>hep^act dronc⁻ disc showing that there is very little apoptosis (Caspase activity) in the spalt domain.