DEV032300 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Six3 protein sequence is highly conserved in the Six3 homeobox, the Six3-specific domain and the groucho-interaction domain. Alignment of Six3 genes from human (Hs; NP_005404), chicken (Gg; NP_989695), African clawed toad (Xl; NP_001079171), zebrafish (Dr; NP_571438), acorn worm (Sk; AAP79281); sea urchin, in bold (Sp; XM_776603.2); fruit fly (Dm; AAD39863); and jellyfish (Pc; AAT11872). Boundaries of the six domain and homeodomain are indicated by magenta and blue arrows and the groucho-interaction domain is boxed.

• Supplemental Figure S2 -

  Fig. S2. In Δcadherin mRNA-injected embryos, Six3 mRNA distributions are absent from vegetal cells but retained throughout the animal hemisphere, reflecting the early expression pattern of this gene prior to restriction by canonical Wnt-dependent processes. Six3 whole-mount in situ hybridization in (A) control 24-hour and (B) Δcadherin-injected embryos.

• Supplemental Figure S3 -

  Fig. S3. Rx and serotonin (sero) are expressed in the same cells in gastrulae. (A-C) Immunostaining of gastrulae for serotonin (green) and in situ hybridization for rx RNA (magenta). Scale bar: 20 µm.