|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
| ||||||||||||||||||||
Files in this Data Supplement:
Fig. S1. ALM migration. ALM cell body distribution in early L1 animals. ALM cell bodies were identified using Nomarski optics and scored relative to the V and P hypodermal nuclei shown in the diagram. Each box lists the percentage of cells found in the region indicated. The black vertical lines delineate the region of most ALMs in wild-type animals, between V2 and V3. The asterisks indicate strains that were scored at 25°C.
Fig. S2. CAN migration. CAN cell body distribution in early L1 animals. CAN cell bodies were identified using Nomarski optics and scored relative to the V and P hypodermal nuclei shown in the diagram. Each box lists the percentage of cells found in the region indicated. The black vertical lines delineate the region of most CANs in wild-type animals, between V3 and V4. The hash sign (#) indicates that both strains used in the RNAi experiment also carried the sensitizing eri-1(mg366) mutation in the genetic background.
Fig. S3. DD process guidance. (A) There are three steps involved in DD process guidance during embryogenesis. (1) After the DD neurons are generated in the ventral nerve cord (VNC), they send processes along the VNC, primarily towards the anterior. (2) The ventral processes branch, forming commissures that extend to the dorsal nerve cord (DNC). (3) In the DNC, the processes branch again anteriorly and posteriorly, forming a ladder-like pattern, as seen in the wild type (fourth image). unc-34 and unc-73 mutants have defects in steps 2 and 3 (fifth image). Anterior is to the left, dorsal is up. (B) Mutation of one copy of crml-1 is sufficient to suppress unc-34 DD process guidance defects. We scored the processes of DD2-DD6 for defects in each animal. The percentage of DD processes that failed to reach the DNC is shown, and was used to compare between strains. All double mutants of crml-1; unc-34 and crml-1/+; unc-34 were statistically different from unc-34 single mutants (***P<0.0002). gm326 and gm331 were also statistically different from gm326/+ and gm331/+ heterozygotes (*P<0.01). unc-73 and unc-73 crml-1 were not statistically different from each other (n.s., not significant). Error bars, standard error. At least 120 axons were scored for each genotype.
| ||||||||||||||||||||
