DEV032847 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression of Coracle in the embryonic CNS. (A-C) Neurexin IV expression and (D-F) Coracle expression are shown in frontal views of stage 15 and stage 16 ventral nerve cords as indicated. Single confocal sections are shown. The orthogonal projection is taken at the position indicated by a broken line in H. (G-I) In the merge, HRP staining (blue) is included for better orientation. (A,B) Increased expression of Neurexin IV in neuronal cells may indicate that in stage 15 embryos Neurexin IV is not entirely recruited to the Wrapper-expressing midline glia, whereas in older embryos (C), most NeurexinIV protein is found at the midline.

• Supplemental Figure S2 -

  Fig. S2. Expression of different Neurexin IV mutants in wild-type embryos. Single confocal sections are shown. (A) The image shows a lateral view of a stage 16 embryo focusing on the dorsal branch of the trachea (tr) and muscle fibers (ms). Expression of a secreted Neurexin IV^exon3-GFP protein (green) is found intercellular spaces and not within the tracheal epithelium. The protein accumulates in the hemolymph, and no binding to specific cell compartments can be observed. (B) Schematic representation of the Neurexin IV protein and the protein variants generated in this study. The localization of the different proteins in epithelial tissues is indicated. (C) Expression of Neurexin IV^exon3-TM-GFP (green) in the hindgut using the enGal4 driver. The C-terminal truncated Neurexin IV protein is ubiquitously distributed in the membrane. See Fig. 4D for the distribution of the wild-type Neurexin IV protein. (D) Expression of Coracle (red). (E) Merge. (F) Expression of Neurexin IV^exon3-TM-GFP (green) in the ventral nerve cord using the elavGal4 driver. (G) Expression of Discs large in the ventral nerve cord. (H) Neuronal membranes are shown following HRP staining (red).