DEV030619 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. E7.25 embryo. Three-hour movie of an E7.25 Stella-GFP embryo, bisected sagittally, and cultured as described in the Materials and methods.

• Movie 2 -

  Movie 2. E7.5 embryo. Twelve-hour movie of an E7.5 Stella-GFP embryo bisected sagittally, and cultured as described in the Materials and methods.

• Movie 3 -

  Movie 3. E7.5 Steel^+/+ embryo. Seven-hour movie of an E7.5 Stella-GFP/Steel^+/+ embryo bisected sagittally, and cultured as described in the Materials and methods.

• Movie 4 -

  Movie 4. E7.5 Steel^+/− embryo. Seven-hour movie of an E7.5 Stella-GFP/Steel^+/− embryo bisected sagittally, and cultured as described in the Materials and methods.

• Movie 5 -

  Movie 5. E7.5 Steel^−/− embryo. Seven-hour movie of an E7.5 Stella-GFP/Steel^−/− embryo bisected sagittally, and cultured as described in the Materials and methods.

• Movie 6 -

  Movie 6. E7.5 Ack2-treated embryo. Six-hour movie of an E7.5 Stella-GFP embryo, bisected sagittally, and cultured in medium containing 10 µg/ml Ack2 blocking antibody as described in the Materials and methods.

• Movie 7 -

  Movie 7. E7.5 IgG control embryo. Seven-hour movie of an E7.5 Stella-GFP embryo, bisected sagittally, and cultured in medium containing 10 µg/ml non-immune mouse IgG as a control.