DEV031203 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Apical cell surface staining of N-cadherin is specific. (A) Dorsal plate cross-section of a stage 14 embryo injected with 100 pg of Xt-N-cad-Myc mRNA into each dorsal animal cell at the 8-cell stage and subsequently stained for the Myc tag. (B) N-cad immunostaining of a stage 14 neural plate depleted of N-cadherin only in one side by injecting 20 ng of the N-cad MO into a single dorsal animal cell at the 8-cell stage. (C,D) En face and cross section views of stage 14 neural plates stained for β-catenin.

• Supplemental Figure S2 -

  Fig. S2. N-cadherin does not rescue the thinning movements of E-cadherin-depleted ventral ectoderm. Quantitation of the mean thicknesses of the ventral ectoderms.

• Supplemental Figure S3 -

  Fig. S3. Control for N-cadherin staining of E-cadherin MO only injected ventral ectoderms. (A) En face view. (B) Cross-section view.

• Movie 1 -

  Movie 1. N-cadherin in the neural plate is required for the morphogenic movements of neurulation. Embryo on the left is an uninjected control, embryo on the right was injected with 20 ng N-cadherin MO into each of the two dorsal animal cells of the 8-cell-stage embryo. Total movie length is 4 hours, showing the beginning to the end of neurulation.

• Movie 2 -

  Movie 2. E-cadherin is required for the spreading movements of the non-neural ectoderm. Embryo on the left is an uninjected control, embryo on the right was injected with 18 ng E-cadherin MO into each of the two ventral animal cells of the 8-cell-stage embryo. Total movie length is 5.5 hours, showing the beginning of neurulation to very early tail bud stage.